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MUSCLE FATIGUE LIGHT GATE EXPERIMENT

Published: Sep-2026 | Category: Secondary Science

How long can you keep moving one finger at the same speed? It sounds easy, but repeated movement soon becomes slower, less regular and more difficult to maintain. This simple biology investigation turns that familiar feeling of muscle fatigue into data students can measure and analyse.

Using a Data Harvest Wireless Light Gate Sensor and EasySense software, students record the speed of repeated finger movements. They can then look for evidence of fatigue and investigate whether warming or cooling the hand changes muscle performance.

What is muscle fatigue?

Muscles create movement by contracting and pulling on bones across joints. These contractions require energy and depend on processes involving the nervous system, blood supply, oxygen and nutrients.

During repeated activity, a muscle may become less able to maintain the same level of performance. This is called muscle fatigue. It does not have a single cause: changes within the muscle, the availability of energy and the nervous system's control of movement can all contribute.

In this investigation, fatigue may appear as a reduction in finger speed, a longer delay between movements or increasingly irregular movement.

Learning objectives

By completing this activity, students can:

  • describe how repeated muscle activity can lead to fatigue;
  • use a Light Gate to measure the speed of a repeated movement;
  • identify patterns in speed and repetition rate over time;
  • compare results collected under different temperature conditions;
  • distinguish between an expected outcome and evidence shown by the data; and
  • evaluate variables that affect the reliability of a human-participant investigation.

Equipment

  • Wireless Light Gate Sensor (1200)
  • EasySense software, version 2 or later
  • A computer or compatible device
  • A book, or a stand and clamp, if needed to position the Light Gate
  • A large beaker or bowl for the temperature extension
  • Hand-warm water and cool water

Setting up the investigation

Connect the Light Gate in EasySense and select Timing. Choose Speed/velocity at A with a Single interrupt card, then enter the depth of the test subject's finger when prompted.

Position the hand so that the chosen finger can move up and down through the Light Gate's infrared beam. The finger should clear the beam at the bottom of each movement, so the Light Gate does not remain triggered when the finger touches the bench. A book, stand or clamp can be used to adjust the sensor height.

A short practice run is useful. The subject should move only the finger, keeping the hand, wrist and arm still. As fatigue develops, it is tempting to compensate by moving the wrist or arm, which would make the comparison less reliable.

Method

  1. Ask the subject to rest their hand comfortably in the agreed position.
  2. Select Start in EasySense.
  3. Ask the subject to move one finger up and down through the infrared beam as quickly as they comfortably can, without moving their wrist or arm.
  4. Continue for about 30 seconds. Fatigue may begin to become noticeable after 10 to 20 seconds.
  5. Select Stop and save the data.
  6. To compare several subjects or conditions on the same display, select Use Overlay before starting the next recording.

What should the data show?

EasySense initially plots speed against reading number. Alternate readings may appear higher and lower because the finger is moving in opposite directions as it enters and leaves the beam. The overall pattern is more important than any one bar.

If the pattern is difficult to see, apply approximately five units of smoothing using the Smoothing tool. A downward trend would show that finger movement is becoming slower as fatigue develops. Smoothing can make the trend clearer, but students should also inspect the original data because smoothing can hide short-term variation.

Speed against reading number does not show the whole story. Change the X-axis to Time to plot speed against time. If the gaps between the bars grow, each repetition is taking longer. This gives students two possible signs of fatigue to consider:

  • a reduction in the speed of each finger movement; and
  • an increase in the time between movements.

Investigating the effect of temperature

Repeat the investigation after the subject has placed their whole hand in hand-warm water for three to four minutes. Repeat it again after using cool water. Keep the movement, finger position, recording time and rest period as consistent as possible.

Before collecting data, students can form a hypothesis. They might predict that cooling the hand will reduce circulation and affect muscle function, so fatigue will appear sooner. They might also predict that gentle warming will help the muscle maintain its performance for longer. The recorded evidence should decide whether the results support these predictions.

Interpreting and evaluating the results

Students can compare the three conditions by asking:

  • How quickly did movement speed begin to decrease?
  • How did the time between repetitions change?
  • Was the movement more irregular in one condition?
  • Do repeated trials show a similar pattern?
  • Does the evidence support the original hypothesis?

Human investigations naturally contain variation. Motivation, dominant hand, starting speed, finger depth, practice, recovery time and small wrist movements could all affect the result. Students should consider how to control these factors and whether percentage change is more useful than comparing raw speeds between different people.

EasySense skills developed

  • Connecting a Wireless Light Gate Sensor
  • Configuring a Timing recording
  • Entering the dimension of the moving object
  • Using overlays to compare trials
  • Applying smoothing to reveal a trend
  • Changing the X-axis from reading number to time
  • Adjusting axis limits to examine the data clearly

Safety and practical considerations

  • Participation should be voluntary, and results should be recorded anonymously where appropriate.
  • This is not a test of strength. The subject should stop immediately if they feel pain, cramp, numbness or unusual discomfort.
  • Use only comfortably hand-warm water, never excessively hot water. Check the temperature before the subject places their hand in it.
  • Use cool rather than painfully cold water, and stop if the subject becomes uncomfortable.
  • Keep water well away from computers and electrical connections, and dry hands thoroughly before touching equipment.

Ideas for further investigation

Students could compare the dominant and non-dominant hand, investigate the effect of a controlled warm-up, or test whether a longer recovery period changes the next trial. Any extension should remain non-invasive, comfortable and designed so that only one variable changes at a time.

Turning movement into evidence

This activity makes an everyday biological experience measurable. By comparing speed, timing and the spacing between movements, students can see that muscle fatigue affects more than how a movement feels. It also provides a useful opportunity to discuss hypotheses, variation, fair testing and the difference between a visible trend and a firm conclusion.

Download the full activity

The complete Muscle fatigue worksheet includes the apparatus and EasySense setup, step-by-step method, temperature comparison, analysis guidance, questions, example graphs, and teacher and technician notes.

Find and download the worksheet in Practical Explorer

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